Part:BBa_K2146006:Design

pWH1266 ori-Ptac-lacOc-ampC ADC-33-T1-lacI-PT5-gusA-T2-pSB1C3

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 561
  Illegal NheI site found at 1351
  Illegal NheI site found at 2689
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 368
  Illegal BamHI site found at 4371
  Illegal XhoI site found at 4218
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 2454
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Four transformed bacteria populations are spread onto the plate in the picture. The media contains ceftazidime and histochemical substrate, X-gluc. All four populations grow on the media, showing that ADC-33 is successfully subcloned into the plasmids used to transform these bacteria. Bacteria containing gusA gene would show blue color. As gusA is an E. coli gene, two populations on the top panels both show blue color. The bottom left panel A. baybyi shows blue colors too, indicating that gusA has been successfully subcloned into the plasmid. The bottom right contains ADC-33 but not gusA, so they grow in white.

Source

n/a